Seroatlas · Human Serome Atlas

ACAT2

Acetyl-CoA acetyltransferase, cytosolic

Also known as: THIC_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BWD1
Gene
ACAT2
Ensembl
ENSG00000120437
Chromosome
6
Canonical length
397 aa
Protein class
Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol
Quaternary structure
Homotetramer

OverviewNCBI Gene

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

Canonical amino-acid sequenceUniProt

397 residues, UniProt reviewed canonical sequence.

>Q9BWD1|ACAT2
     1  MNAGSDPVVI VSAARTIIGS FNGALAAVPV QDLGSTVIKE VLKRATVAPE DVSEVIFGHV
    61  LAAGCGQNPV RQASVGAGIP YSVPAWSCQM ICGSGLKAVC LAVQSIGIGD SSIVVAGGME
   121  NMSKAPHLAY LRTGVKIGEM PLTDSILCDG LTDAFHNCHM GITAENVAKK WQVSREDQDK
   181  VAVLSQNRTE NAQKAGHFDK EIVPVLVSTR KGLIEVKTDE FPRHGSNIEA MSKLKPYFLT
   241  DGTGTVTPAN ASGINDGAAA VVLMKKSEAD KRGLTPLARI VSWSQVGVEP SIMGIGPIPA
   301  IKQAVTKAGW SLEDVDIFEI NEAFAAVSAA IVKELGLNPE KVNIEGGAIA LGHPLGASGC
   361  RILVTLLHTL ERMGRSRGVA ALCIGGGMGI AMCVQRE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACAT2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.22
Highest tissue expression
216 nTPM

Expression across tissuesHPA

Tissue

  • liver: 216 nTPM
  • esophagus: 64 nTPM
  • spinal cord: 50 nTPM
  • cerebral cortex: 45 nTPM
  • epididymis: 38 nTPM
  • thymus: 37 nTPM

Single-cell type

  • early spermatids: 248 nCPM
  • breast lactating cells: 236 nCPM
  • esophageal suprabasal cells: 224 nCPM
  • alveolar cells type 2: 214 nCPM
  • esophageal apical cells: 198 nCPM
  • late spermatids: 190 nCPM

Immune cell

  • T-reg: 79 nTPM
  • memory CD4 T-cell: 54 nTPM
  • naive CD4 T-cell: 54 nTPM
  • naive B-cell: 52 nTPM
  • gdT-cell: 48 nTPM
  • memory CD8 T-cell: 48 nTPM

Brain region

  • pons: 55 nTPM
  • medulla oblongata: 41 nTPM
  • cerebellum: 36 nTPM
  • white matter: 31 nTPM
  • hypothalamus: 31 nTPM
  • midbrain: 27 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.14
gnomAD pLI
0
gnomAD missense Z
0.7
DepMap mean gene effect
0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ACAT2 as an antibody target. Whether an autoantibody or antibody against ACAT2 could matter depends on whether native ACAT2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ACAT2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ACAT2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ACAT2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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