ACADL

Protein identityUniProt · HPA

UniProt accession

P28330

Gene

ACADL

Ensembl

ENSG00000115361

Chromosome

2

Canonical length

430 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Quaternary structure

Homotetramer

OverviewNCBI Gene

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

430 residues, UniProt reviewed canonical sequence.

>P28330|ACADL
     1  MAARLLRGSL RVLGGHRAPR QLPAARCSHS GGEERLETPS AKKLTDIGIR RIFSPEHDIF
    61  RKSVRKFFQE EVIPHHSEWE KAGEVSREVW EKAGKQGLLG VNIAEHLGGI GGDLYSAAIV
   121  WEEQAYSNCS GPGFSIHSGI VMSYITNHGS EEQIKHFIPQ MTAGKCIGAI AMTEPGAGSD
   181  LQGIKTNAKK DGSDWILNGS KVFISNGSLS DVVIVVAVTN HEAPSPAHGI SLFLVENGMK
   241  GFIKGRKLHK MGLKAQDTAE LFFEDIRLPA SALLGEENKG FYYIMKELPQ ERLLIADVAI
   301  SASEFMFEET RNYVKQRKAF GKTVAHLQTV QHKLAELKTH ICVTRAFVDN CLQLHEAKRL
   361  DSATACMAKY WASELQNSVA YDCVQLHGGW GYMWEYPIAK AYVDARVQPI YGGTNEIMKE
   421  LIAREIVFDK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACADL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

26 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.16

gnomAD pLI

0

gnomAD missense Z

0.86

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• carnitine metabolic process, CoA-linked
• fatty acid beta-oxidation using acyl-CoA dehydrogenase
• lipid catabolic process
• long-chain fatty acid catabolic process
• negative regulation of fatty acid biosynthetic process
• negative regulation of fatty acid oxidation
• positive regulation of cold-induced thermogenesis
• regulation of cholesterol metabolic process
• temperature homeostasis
• carnitine catabolic process

Molecular functions

• flavin adenine dinucleotide binding
• long-chain fatty acyl-CoA dehydrogenase activity
• protein homodimerization activity

Cellular components

• cytoplasm
• mitochondrial matrix
• mitochondrial membrane
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Acyl-CoA dehydrogenase, conserved site
• Acyl-CoA dehydrogenase/oxidase, middle domain
• Acyl-CoA dehydrogenase/oxidase, C-terminal
• Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily
• Acyl-CoA dehydrogenase/oxidase, N-terminal
• Acyl-CoA dehydrogenase-like, C-terminal
• Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily
• Acyl-CoA oxidase/dehydrogenase, middle domain superfamily
• Acyl-CoA dehydrogenase, C-terminal domain
• Acyl-CoA dehydrogenase, middle domain
• Acyl-CoA dehydrogenase, N-terminal domain
• Long-chain specific acyl-CoA dehydrogenase

KeywordsUniProt

• Acetylation
• FAD
• Fatty acid metabolism
• Flavoprotein
• Lipid metabolism
• Mitochondrion
• Oxidoreductase
• Phosphoprotein
• Transit peptide