AADAT

Protein identityUniProt · HPA

UniProt accession

Q8N5Z0

Gene

AADAT

Ensembl

ENSG00000109576

Chromosome

4

Canonical length

425 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Vesicles,Plasma membrane

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Canonical amino-acid sequenceUniProt

425 residues, UniProt reviewed canonical sequence.

>Q8N5Z0|AADAT
     1  MNYARFITAA SAARNPSPIR TMTDILSRGP KSMISLAGGL PNPNMFPFKT AVITVENGKT
    61  IQFGEEMMKR ALQYSPSAGI PELLSWLKQL QIKLHNPPTI HYPPSQGQMD LCVTSGSQQG
   121  LCKVFEMIIN PGDNVLLDEP AYSGTLQSLH PLGCNIINVA SDESGIVPDS LRDILSRWKP
   181  EDAKNPQKNT PKFLYTVPNG NNPTGNSLTS ERKKEIYELA RKYDFLIIED DPYYFLQFNK
   241  FRVPTFLSMD VDGRVIRADS FSKIISSGLR IGFLTGPKPL IERVILHIQV STLHPSTFNQ
   301  LMISQLLHEW GEEGFMAHVD RVIDFYSNQK DAILAAADKW LTGLAEWHVP AAGMFLWIKV
   361  KGINDVKELI EEKAVKMGVL MLPGNAFYVD SSAPSPYLRA SFSSASPEQM DVAFQVLAQL
   421  IKESL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AADAT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

63 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0.15

gnomAD missense Z

2.35

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• 2-oxoglutarate metabolic process
• glutamate metabolic process
• kynurenine metabolic process
• L-lysine catabolic process to acetyl-CoA via saccharopine
• alpha-amino acid metabolic process

Molecular functions

• kynurenine-glyoxylate transaminase activity
• kynurenine-oxoglutarate transaminase activity
• protein homodimerization activity
• pyridoxal phosphate binding
• 2-aminoadipate transaminase activity
• glycine:2-oxoglutarate aminotransferase activity
• methionine-glyoxylate transaminase activity

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Aminotransferase, class I/classII, large domain
• Pyridoxal phosphate-dependent transferase, major domain
• Pyridoxal phosphate-dependent transferase
• Aminotransferase class I and II
• Class-I pyridoxal-phosphate-dependent aminotransferase-like

KeywordsUniProt

• Acetylation
• Aminotransferase
• Mitochondrion
• Pyridoxal phosphate
• Transferase
• Transit peptide