Seroatlas · Human Serome Atlas

A4GNT

Alpha-1,4-N-acetylglucosaminyltransferase

Also known as: A4GCT_HUMAN, alpha4GnT

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UNA3
Gene: A4GNT
Ensembl: ENSG00000118017
Chromosome: 3
Canonical length: 340 aa
Protein class: Metabolic proteins, Predicted intracellular proteins
Secretome location: Intracellular and membrane

OverviewNCBI Gene

This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

340 residues, UniProt reviewed canonical sequence.

>Q9UNA3|A4GNT
     1  MRKELQLSLS VTLLLVCGFL YQFTLKSSCL FCLPSFKSHQ GLEALLSHRR GIVFLETSER
    61  MEPPHLVSCS VESAAKIYPE WPVVFFMKGL TDSTPMPSNS TYPAFSFLSA IDNVFLFPLD
   121  MKRLLEDTPL FSWYNQINAS AERNWLHISS DASRLAIIWK YGGIYMDTDV ISIRPIPEEN
   181  FLAAQASRYS SNGIFGFLPH HPFLWECMEN FVEHYNSAIW GNQGPELMTR MLRVWCKLED
   241  FQEVSDLRCL NISFLHPQRF YPISYREWRR YYEVWDTEPS FNVSYALHLW NHMNQEGRAV
   301  IRGSNTLVEN LYRKHCPRTY RDLIKGPEGS VTGELGPGNK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against A4GNT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.28
Highest tissue expression: 22 nTPM

Expression across tissuesHPA

Tissue

stomach: 22 nTPM
pancreas: 3 nTPM
duodenum: 2.5 nTPM
gallbladder: 1.8 nTPM
kidney: 1.1 nTPM
retina: 0.8 nTPM

Single-cell type

mucous neck cells: 51 nCPM
gastric chief cells: 27 nCPM
pancreatic duct cells: 6.1 nCPM
cholangiocytes: 5.9 nCPM
foveolar cells: 2.9 nCPM
parietal cells: 1.8 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 1.1 nTPM
midbrain: 1.1 nTPM
medulla oblongata: 0.7 nTPM
spinal cord: 0.7 nTPM
pons: 0.4 nTPM
cerebral cortex: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.74
gnomAD pLI: 0.17
gnomAD missense Z: 0.24
DepMap mean gene effect: 0.03
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

acetylglucosaminyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads A4GNT as an antibody target. Whether an autoantibody or antibody against A4GNT could matter depends on whether native A4GNT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

A4GNT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label A4GNT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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